rhigf 2 (R&D Systems)

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R&D Systems rhigf 2
Rhigf 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhigf+ii/Recombinant+Human+IGF-II%2FIGF2+Protein%2C+CF/pmc10931042-76-27-29
Average 93 stars, based on 69 article reviews

rhigf 2 - by Bioz Stars, 2026-09

93/100 stars

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Translocation Assay:

Article Title: The IGF2 mRNA binding protein p62/IGF2BP2-2 induces fatty acid elongation as a critical feature of steatosis
Article Snippet: .. For the detection of IGF2-mediated SREBF1 translocation, cells were treated for the indicated time with rhIGF-II (0.075 μg/ml, 292-G2; R&D Systems, Minneapolis, MN) or with IGF2 antibody (ab9574; Abcam, Cambridge, UK) as previously described ( 27 ). ..

Article Title: The IGF2 mRNA binding protein p62/IGF2BP2-2 induces fatty acid elongation as a critical feature of steatosis
Article Snippet: .. For the detection of IGF2-mediated SREBF1 translocation, cells were treated for the indicated time with rhIGF-II (0.075 g/ml, 292-G2; R&D Systems, Minneapolis, MN) or with IGF2 antibody (ab9574; Abcam, Cambridge, UK) as previously described ( 27 ). ..

Fractionation:

Article Title: The Role of SOX9 in IGF-II-Mediated Pulmonary Fibrosis.
Article Snippet: .. Once 70–90% confluent, the media was changed to serum free DMEM with 1% AB/AM for 12 to 24 h before stimulation with vehicle/PBS (Cat#21-040-CV, Corning, Corning, NY, USA) or 200 ng/mL rhIGF-II (Cat# 292-G2, R&D Systems, Minneapolis, MN, USA) for 2 and 4 h. Cells were harvested with trypsin (Cat#25-053-CI, Corning, Corning, NY, USA),and fractionations were performed using the subcellular protein fractionation kit for cultured cells (Cat# 78840, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. The subcellular fractions were supplemented with a protease inhibitor cocktail (included in the kit) and sodium orthovanadate (Cat#J60191, Alfa Aesar by Thermo Fisher Scientific, Haverhill, MA, USA).

Cell Culture:

other:

Article Title: The Role of SOX9 in IGF-II-Mediated Pulmonary Fibrosis.
Article Snippet: Fibroblasts were stimulated with vehicle/PBS (Cat#21-040-CV, Corning, Corning, NY, USA) or 200 ng/mL rhIGF-II (Cat# 292-G2, R&D Systems, Minneapolis, MN, USA) for the times indicated in the figure legends.

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